Isolation and characterization of butanol-resistant mutants of Clostridium acetobutylicum.

نویسندگان

  • M Hermann
  • F Fayolle
  • R Marchal
  • L Podvin
  • M Sebald
  • J P Vandecasteele
چکیده

In a wild-type strain of Clostridium acetobutylicum isolated from soil, solvent production appeared limited by butanol toxicity. Butanol-resistant mutants have been obtained which produced significantly higher solvent concentrations (about 30%) than the wild-type strain. Some other physiological differences were observed between a selected resistant mutant and the wild-type strain at the level of solvent resistance and sporulation.

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Transposon mutagenesis of Clostridium acetobutylicum P262: isolation and characterization of solvent deficient and metronidazole resistant mutants.

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UNLABELLED Engineering industrial microorganisms for ambitious applications, for example, the production of second-generation biofuels such as butanol, is impeded by a lack of knowledge of primary metabolism and its regulation. A quantitative system-scale analysis was applied to the biofuel-producing bacterium Clostridium acetobutylicum, a microorganism used for the industrial production of sol...

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 50 5  شماره 

صفحات  -

تاریخ انتشار 1985